引言 染色体作为遗传物质的主要载体,其数目和结构的研究是细胞遗传学的重要内容。通过有效的方法,制得理想的染色体标本,将为柑桔遗传育种研究提供一些重要的信息,有助于柑桔细胞遗传学研究的发展。在柑桔上,以前大多采用醋酸洋红染色、苏木 Introduction Chromosomes as the main carrier of genetic material, the number and structure of the study is an important part of cytogenetics. Through the effective method to obtain the ideal chromosome specimen, it will provide some important information for citrus genetic breeding research and contribute to the development of citrus cytogenetics. In oranges, most of the previous use of acetate magenta staining, hematoxylin