介绍一种在低温生物学规律及中耳移植的生物学特性基础上发展起来的鼓膜-听小骨移植材料分次低温处理新方法。即分别从50只雄性白鼠的两侧取出颞骨、肋骨,分离出鼓膜-听小骨系统,肋软骨及肋骨。一套组织为鼓膜-听小骨系统,另一套弹性软骨取自于耳廓及肋骨。这些材料一半为对照组(25套为新鲜组织,25套用作单次冷冻保存),另一半50 A new method of fractionating cryogenic treatment of eardrum-to-bony transplanted material was developed based on the biological laws of hypothermia and the biological characteristics of middle ear. That is, 50 male rats were removed from both sides of the temporal bone, ribs, isolated from the tympanic membrane - the ossicular system, costal cartilage and ribs. One set of tissue is the tympanic membrane - the ossicle system and the other set of elastic cartilage is taken from the auricles and ribs. Half of these materials were control groups (25 for fresh tissue, 25 for single cryopreservation) and the other half 50