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目的了解 N-ras 基因突变,APC 和 DCC 基因杂合性缺失在原发肝癌发生发展中的作用。方法采用聚合酶链反应-限制性片段长度多态现象(PCR-RFLP)分析技术,对30例外科手术切除原发肝细胞癌组织中癌基因 N-ras第12位密码子突变,抑癌基因 APC 和 DCC 杂合缺失(LOH)进行研究。结果原发肝癌中 N-ras 第12位密码子的突变率为16.7%(5/30),APC 和 DCC 基因 LOH 率分别为36.4%(8/22)和21.4%(6/28)。N-ras 基因突变、APC 和 DCC 基因 LOH与血清 AFP 水平,有无肝硬化、肿瘤大小、分化程度,有无转移和 HBV 感染无显著相关(p值<0.05).结论 N-ras基因突变,APC 和 DCC 基因 LOH 可能参与了原发肝癌的发生和发展.
Objective To understand the role of N-ras gene mutation, loss of heterozygosity of APC and DCC genes in the development of primary liver cancer. Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis technique was used to detect the mutation of the 12th codon in oncogene N-ras in 30 cases of primary hepatocellular carcinoma. Loss of heterozygosity of APC and DCC (LOH) was studied. Results The mutation rate of N-ras codon 12 in primary liver cancer was 16.7% (5/30). The LOH rates of APC and DCC were 36.4% (8/22) and 21.4% (6/28), respectively. N-ras gene mutation, APC and DCC gene LOH and serum AFP levels, with or without liver cirrhosis, tumor size, differentiation degree, metastasis and HBV infection were not significantly correlated (p<0.05). Conclusion N-ras gene mutation, APC and DCC gene LOH may be involved in the occurrence and development of primary liver cancer.