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目的 探讨γ射线对白血病细胞中MMP - 2表达的影响 ,以及从细胞水平初步探讨牛磺酸对肿瘤细胞受辐照后MMP - 2表达的抑制作用。方法 6 0 Coγ射线 1 5Gy照射白血病细胞K562作为阳性对照组 ,不照射的作为阴性对照组 (1 -不加药 ,2 -加药 2 0 0mg/L) ,另设三组实验组 (照射 ,加药量分别为 50mg/L、1 0 0mg/L、2 0 0mg/L) ,照后 1 2h收集 ,细胞经处理后采用Western blotting技术分析各组细胞MMP - 2的表达水平。结果 1 5Gy照射剂量阳性对照组细胞MMP - 2表达量明显增加 ,阴性对照组 1中MMP - 2表达量较少 ,实验组细胞MMP - 2表达量随剂量增加而依次减少 ,其中 2 0 0mg/L组与阴性对照组 1细胞MMP - 2表达量基本一致。结论 1 5Gy6 0 Coγ射线照射促进白血病细胞K562中MMP - 2表达 ,电离辐射可引起肿瘤细胞的进一步侵袭和转移 ;加不同剂量牛磺酸后对MMP - 2表达有抑制作用 ,且与剂量呈相关性 ,实现生物防治的作用
Objective To investigate the effect of γ - rays on the expression of MMP - 2 in leukemia cells and to investigate the inhibitory effect of taurine on the expression of MMP - 2 after exposure to tumor cells at the cellular level. Methods Sixty Co γ-rays irradiated with 5Gy leukemia cells K562 were used as a positive control group, without irradiation as a negative control group (1 - without drug, 2 - with drug 200 mg / L) The dosage was 50mg / L, 100mg / L, 200mg / L respectively). The cells were harvested at 12h after irradiation. The cells were treated and the expression of MMP - 2 was analyzed by Western blotting. Results The expression of MMP - 2 in the positive control group was significantly increased at 5 Gy irradiation, while the expression of MMP - 2 in the negative control group 1 was less. The expression of MMP - 2 in the experimental group decreased with the increase of the dosage, The expression of MMP - 2 in L group and negative control group was basically the same. Conclusion 5 Gy 60Coγ ray can promote the expression of MMP - 2 in K562 leukemia cells. Ionizing radiation can cause further invasion and metastasis of tumor cells. After adding different doses of taurine, the expression of MMP - 2 is inhibited and dose dependent Sex, to achieve the role of biological control