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应用生物素标记人乳头瘤病毒（ＨＰＶ）６、１１、１６、１８型探针斑点杂交检查４８例宫颈癌标本，其中１５例ＨＰＶ１６慢性（３１％）、其余均阴性。又对该组标本中３４份进行聚合酶链反应（ＰＣＲ），１４例ＨＰＶ１６阳性（４１％）、２例ＨＰＶ１８阳性（６％）。另外ＰＣＲ检查宫颈刮片正常者２０例，１例ＨＰＶ１６阳性（５％）。本研究提示ＰＣＲ敏感性高于斑点杂交。对ＨＰＶ检查结果解释应慎重，单独ＨＰＶ检查不能肯定是否患宫颈癌，需结合其他检查。ＨＰＶ１６、１８型阳性病人应定期检查以早期发现宫颈癌。
Forty-eight specimens of cervical cancer were examined by dot blot hybridization with biotin-labeled human papillomavirus (HPV) 6,11,16,18 probes, of which 15 were negative for HPV16 (31%). Polymerase chain reaction (PCR) was performed on 34 of the samples in this group, 14 HPV16 positive (41%) and 2 HPV18 positive (6%). In addition, there were 20 cases of normal cervical smear by PCR and 1 case of HPV16 positive (5%). This study suggests that PCR is more sensitive than dot blot. HPV test results should be interpreted cautiously, HPV test alone can not be sure whether suffering from cervical cancer, the need to combine other tests. HPV16,18 positive patients should be regularly checked to detect cervical cancer early.