Eight-year-old “ Shixia” longan( Dimocarpus longan Loureiro) trees were treated under low temperature stress,oxidative stress,and natural low temperature conditions + experimental agents to investigate the relationship between oxidative damage and flowering of longan. The results indicated that oxidative stress was conducive to flowering of longan,which also led to accumulation of reactive oxygen species in various tissues of longan. The detection results of reactive oxygen species,ascorbic acid content and MDA content in apical buds and leaves showed that ascorbic acid could inhibit flowering of longan,while MDA could promote flowering of longan. Therefore,there was a positive correlation between reactive oxygen species and flowering of longan. Eight-year-old “Shixia” longan (Dimocarpus longan Loureiro) trees were treated under low temperature stress, oxidative stress, and natural low temperature conditions + experimental agents to investigate the relationship between oxidative damage and flowering of longan. The results shows that oxidative stress was conducive to flowering of longan, which also led to accumulation of reactive oxygen species in various tissues of longan. The detection results of reactive oxygen species, ascorbic acid content and MDA content in apical buds and leaves showed that ascorbic acid could inhibit flowering of longan, while MDA could promote flowering of longan. Therefore, there was a positive correlation between reactive oxygen species and flowering of longan.