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In the present study, splenocytes from oneBalb/c mouse immunized with syngeneic mono-clonal anti-HBs(Ab_1)were fused with SP2/0myeloma cells at the ratio of 5:1 and culturedin the presence of HAT. Ten days after fusion,86% of the culture wells showed the growing ofcell clones. With ELISA screening the culturalsupernatant, 18% and 16% of the clone-growing
In the present study, splenocytes from one Balb / c mouse immunized with syngeneic mono-clonal anti-HBs (Ab_1) were fused with SP2 / 0 myeloma cells at the ratio of 5: 1 and cultured in the presence of HAT. Ten days after fusion, 86 % of the culture wells showed the growing of clones. With ELISA screening the cultural supernatant, 18% and 16% of the clone-growing