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B663的抗麻风作用已充分肯定,但其作用方式基本上仍不知道。作者曾报告B663在试管内浓度接近血清治疗水平时则增加溶酶体酶的合成和增加非刺激的小鼠腹膜巨噬细胞吞噬标记的免疫复合物的数量。本研究报告B663对取自用各种浓度B663管饲法喂食小鼠的巨噬细胞的作用。作者对三组小鼠用B663 0.1mg、1mg和10mg/kg/d喂食3周,每日B663系溶于0.3ml葵花油内管饲,对照组仅管饲葵花油。在实验开始时和每周一次共3周,将小鼠吸入氯仿处死,取腹膜细胞作巨噬细胞培养。结果:B663对溶酶体酶水平的影响取自用B6631 mg和10mg/kg/d管饲21天的小鼠非刺激的腹膜巨噬细胞显示所检测的三种溶酶体酶-N-乙酰-β-
The anti-leprosy effect of B663 has been fully affirmed, but its mode of action is still basically unknown. The authors have reported that B663 increases lysosomal enzyme synthesis and increases the number of phagocytosed labeled immune complexes in non-stimulated mouse peritoneal macrophages when in vitro concentrations near serum treatment levels. This study reports the effect of B663 on macrophages taken from mice fed gavage at various concentrations of B663. The authors of three groups of mice fed with B663 0.1mg, 1mg and 10mg / kg / d for 3 weeks, daily B663 dissolved in 0.3ml sunflower oil tube feeding, the control group only tube sunflower oil. At the beginning of the experiment and once a week for a total of 3 weeks, mice were killed by inhalation of chloroform and peritoneal cells were harvested for macrophage culture. Results: Effect of B663 on Lysosomal Enzyme Levels Non-stimulated peritoneal macrophages from mice gavaged with B6631 mg and 10 mg / kg / d for 21 days showed that the three lysosomal enzymes N-acetyl -β-