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目的:探讨缺氧诱导因子-1α(HIF-1α)基因沉默对裸鼠肺癌移植瘤放射敏感性的影响。方法:采用A549和A549/HIF-1α(-)细胞株建立人肺癌移植瘤模型,分别给予5、10、15和20Gy单次剂量X射线照射,观察放射治疗对肿瘤的抑制作用,根据肿瘤生长延缓和照射剂量分析肿瘤剂量效应及HIF-1α基因沉默对在体肿瘤放射敏感性的影响。结果:放射治疗能明显抑制肿瘤生长,并观察到随照射剂量的增加肿瘤生长延缓愈显著,接受等剂量照射HIF-1α基因沉默肿瘤生长延缓更明显,根据肿瘤剂量效应曲线计算放射增敏比分别为1.40、1.34和1.31。结论:放射治疗能显著抑制A549和A549/HIF-1α(-)肿瘤生长,A549/HIF-1α(-)肿瘤对放射治疗更敏感。
Objective: To investigate the effect of hypoxia inducible factor-1α (HIF-1α) gene silencing on the radiosensitivity of lung cancer xenografts in nude mice. Methods: Human lung cancer xenograft model was established by A549 and A549 / HIF-1α (-) cell lines. The single dose X-ray irradiation of 5, 10, 15 and 20 Gy were given respectively to observe the inhibitory effect of radiotherapy on the tumor. Delayed and Irradiated Dose Analysis of Tumor Dose Effect and HIF-1α Gene Silencing on Radiosensitivity in Tumor of the Body. Results: Radiation therapy can significantly inhibit tumor growth, and observed with the increase of radiation dose, the more obvious the tumor growth retardation, the delay of tumor growth was more obvious when the HIF-1α gene was silenced by equal dose irradiation, and the radiosensitivity ratio was calculated according to the tumor dose-response curve 1.40, 1.34 and 1.31. Conclusion: Radiation therapy can significantly inhibit the growth of A549 and A549 / HIF-1α (-) tumors. A549 / HIF-1α (-) tumors are more sensitive to radiation therapy.