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目的 :探讨倍氯米松对变应性鼻炎鼻分泌物中多种炎性活细胞的作用机制。方法 :采用激光共聚焦显微镜扫描和荧光技术 ,观察变应性鼻炎鼻分泌物中嗜酸性粒细胞、中性粒细胞和淋巴细胞经倍氯米松处理后细胞内的形态变化。结果 :经倍氯米松处理后 ,嗜酸性粒细胞、中性粒细胞组细胞内RNA的荧光染色均减弱 ,RNA/DNA比值均变小 ,嗜酸性粒细胞组差异具有非常显著性意义 (P <0 .0 0 1) ;中性粒细胞组差异具有显著性意义 (P <0 .0 1) ;淋巴细胞组差异无显著性意义 (P >0 .0 5 )。结论 :倍氯米松能够明显地减少嗜酸性粒细胞和中性粒细胞细胞内RNA的含量 ,即减少细胞内RNA的合成和 (或 )促进RNA的降解 ;不直接影响淋巴细胞内的RNA代谢。
Objective: To investigate the mechanism of beclomethasone on multiple inflammatory cells in nasal secretions of allergic rhinitis. Methods: The morphological changes of eosinophils, neutrophils and lymphocytes in nasal secretions of allergic rhinitis treated with beclomethasone were observed by laser scanning confocal microscope and fluorescence technique. Results: After treated with beclomethasone, the staining of RNA in eosinophils and neutrophils decreased, and the ratio of RNA / DNA decreased. The difference of eosinophils group was significant (P < There was no significant difference between lymphocyte group (P> 0.05). The difference of neutrophil group was significant (P <0.01). Conclusion: Beclomethasone can significantly reduce the intracellular RNA content of eosinophils and neutrophils, that is, reduce the intracellular RNA synthesis and / or promote the degradation of RNA; it does not directly affect the RNA metabolism in lymphocytes.