论文部分内容阅读
目的探讨芪棱汤对大鼠局灶性脑缺血再灌注损伤后微管相关蛋白-2(MAP-2)表达的影响及其神经保护作用机制。方法将大鼠随机分为正常组、假手术组、模型组、补阳还五汤组(对照组)及芪棱汤组(治疗组)。复制大鼠局灶性脑缺血再灌注模型并给予相应药物。在再灌注不同时点采用免疫组织化学法测MAP-2表达。结果正常组、假手术组可见MAP-2广泛表达;模型组、对照组及治疗组梗死中心区和周围区在再灌注即刻均出现表达下降,中心区随着再灌注时间延长持续下降,而梗死周围区在再灌注24h降至最低后表达有所上升;治疗组可增加MAP-2表达、促进其修复,以再灌注24h及48h明显,与模型组有明显差异,对照组在48hMAP-2表达亦增加,而治疗组效果更显著。结论芪棱汤能减少MAP-2丢失和促进其修复,具有良好的神经保护作用。
Objective To investigate the effect of Qileng Decoction on the expression of microtubule-associated protein-2 (MAP-2) in rats with focal cerebral ischemia-reperfusion injury and its neuroprotective mechanism. Methods Rats were randomly divided into normal group, sham operation group, model group, Buyang Huanwu Decoction group (control group) and Qileng Decoction group (treatment group). Rat models of focal cerebral ischemia and reperfusion were duplicated and corresponding drugs were given. MAP-2 expression was measured by immunohistochemistry at different time points of reperfusion. RESULTS: MAP-2 was widely expressed in normal and sham-operated groups. The infarct center and surrounding areas of the model group, control group, and treatment group all showed decreased expression at the time of reperfusion, and the central area continued to decrease as reperfusion time prolonged. The expression of MAP-2 was increased and the repair was promoted in the treatment group after 24 hours of reperfusion. The expression of MAP-2 was significantly increased in the treatment group, which was significantly different from that in the model group at 24h and 48h after reperfusion. The expression of MAP-2 in the control group at 48h. Also increased, while the treatment group was more effective. Conclusion Qileng Decoction can reduce the loss of MAP-2 and promote its repair, and has a good neuroprotective effect.