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目的：探讨一氧化氮、内皮素和循环内皮细胞在支气管哮喘中的作用。方法：哮喘患儿６１例，４０名健康儿童为对照，测定其血浆ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和循环内皮细胞（ＣＥＣ）水平，用过敏性哮喘豚鼠进行验证。结果：哮喘患儿血浆ＮＯ－２／ＮＯ－３、ｃＧＭＰ和ＣＥＣ水平均明显升高（Ｐ＜０．０５～００１），其中重度哮喘患儿血浆ＮＯ－２／ＮＯ－３和ＣＥＣ水平较轻中度哮喘患儿更高（Ｐ均＜００１）；重度哮喘患儿血浆ＥＴ水平明显升高（Ｐ＜００１）；哮喘患儿血浆ＮＯ－２／ＮＯ－３与ｃＧＭＰ和ＣＥＣ水平呈显著正相关（Ｐ＜００５～００１）。过敏性哮喘豚鼠ＢＡＬＦ中ＮＯ－２／ＮＯ－３、ＥＴ及ｃＧＭＰ水平均明显升高（Ｐ均＜００１）；血浆中ｃＧＭＰ和ＣＥＣ水平亦升高（Ｐ均＜００５）；糖皮质激素可降低ＮＯ－２／ＮＯ－３、ＥＴ、ｃＧＭＰ和ＣＥＣ水平。结论：哮喘时ＮＯ－２／ＮＯ－３、ＥＴ和ＣＥＣ产生过多，它们在哮喘发病机制中占有重要地位，而且血浆ＥＴ和ＣＥＣ水平可能与哮喘病情相关。
Objective: To investigate the role of nitric oxide, endothelin and circulating endothelial cells in bronchial asthma. Methods: The levels of NO-2 / NO-3, ET, cGMP and circulating endothelial cells (CEC) were measured in 61 asthmatic children and 40 healthy children. Results: The levels of plasma NO-2 / NO-3, cGMP and CEC were significantly increased in children with asthma (P <0.05-0.01). The levels of NO-2 / NO-3 and CEC The levels of plasma ET-1 in children with severe asthma were significantly higher (P <001); the levels of NO-2 / NO-3 and cGMP in children with asthma were significantly higher than those in mild to moderate asthma CEC level was significantly positive (P <0 05 ~ 0 01). The levels of NO-2 / NO-3, ET and cGMP in BALF were significantly increased in allergic asthmatic guinea pigs (all P <001), while the levels of plasma cGMP and CEC were also increased (all P <005) Corticosteroids reduced NO-2 / NO-3, ET, cGMP and CEC levels. CONCLUSION: Excessive production of NO-2 / NO-3, ET and CEC in asthma plays an important role in the pathogenesis of asthma. Moreover, the levels of plasma ET and CEC may be related to the severity of asthma.