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采用免疫组化 L SAB法及图像分析技术 ,定量检测 41例细支气管肺泡癌 (BAC)组织中的神经元特异性烯醇化酶 (NSE)和 α1 -抗胰蛋白酶 (α1 - AT)的表达 ,探讨其与 BAC的组织发生及其生物学行为的关系。结果表明 ,NSE和 α1 - AT阳性率分别为 70 .7% (2 9/ 41)和 85 .4% (35 / 41) ,α1 - AT的表达的强弱与组织亚型有关 ,肺泡型表达强度高于其他三型 (P<0 .0 5 ) ,乳头状型高于粘液型和硬化型 (P<0 .0 5 ) ,粘液型和硬化型间无明显差异 (P>0 .0 5 ) ,NSE表达的强弱与组织亚型无关 (P>0 .0 5 ) ;淋巴结转移组 NSE表达的强度 (0 .10± 0 .0 2 )明显高于无转移组 (0 .0 7±0 .0 2 ) ,淋巴结转移组 α1 - AT表达的强度 (0 .13± 0 .0 2 )则明显低于无转移组 (0 .15± 0 .0 2 ) ,差异均有显著性 (P<0 .0 1)。提示 BAC也具有产生 α1 - AT的能力并大部分伴神经内分泌分化 ,NSE和 α1 - AT表达的强弱与 BAC的生物学行为密切相关
The expression of neuron - specific enolase (NSE) and α1 - antitrypsin (α1 - AT) in 41 patients with bronchial alveolar carcinoma (BAC) was quantitatively detected by immunohistochemical L SAB method and image analysis technique. To explore its relationship with the occurrence of BAC and its biological behavior. The results showed that the positive rates of NSE and α1 - AT were 70.7% (29/41) and 85.4% (35/41), respectively. The expression of α1 - AT was related to the tissue subtype and the expression of alveolar type (P <0.05), the papillary type was higher than mucinous and sclerotic type (P <0.05), and there was no significant difference between mucus type and sclerotic type (P> 0.05) ). The intensity of NSE expression was not related to the tissue subtype (P> 0.05). The intensity of NSE expression in lymph node metastasis group was significantly higher than that in non-metastasis group (0.07 ± 0. 0 2) 0 .0 2), the intensity of α1 - AT expression in lymph node metastasis (0 .13 ± 0. 02) was significantly lower than that in non - metastasis group (0 .15 ± 0. <0 .0 1). It is suggested that BAC also has the ability to produce α1 - AT and most of them have neuroendocrine differentiation. The expression of NSE and α1 - AT is closely related to the biological behavior of BAC