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目的 对痘苗病毒表达载体 p MJ6 0 1进行转化与鉴定 ,为进一步实施痘苗病毒为载体的基因治疗作必要的准备。方法 利用感受态细胞的制备及转化、质粒抽提、琼脂糖凝胶电泳、限制性内切酶酶切等多种基因工程技术对质粒 p MJ6 0 1进行转化及鉴定。结果 琼脂糖凝胶电泳清楚地显示了 p MJ6 0 1质粒 3条带和 Bam HI或 H ind 酶切后的线性条带。结论 痘苗病毒表达载体 p MJ6 0 1的转化与鉴定 ,为痘苗病毒载体系统的基因治疗打下了坚实的基础。
Objective To transform and identify the vaccinia virus expression vector p MJ6 0 1 and make necessary preparations for further gene therapy of vaccinia virus vector. Methods The plasmid p MJ6 0 1 was transformed and identified by using a variety of genetic engineering technologies such as preparation and transformation of competent cells, plasmid extraction, agarose gel electrophoresis and restriction endonuclease digestion. Results The agarose gel electrophoresis clearly showed a linear band of p MJ6 0 1 plasmid 3 and Bam HI or H ind digestion. Conclusion The transformation and identification of vaccinia virus expression vector p MJ6 0 1 laid a solid foundation for the gene therapy of vaccinia virus vector system.