论文部分内容阅读
目的研究海洋胶原肽(MCPs)对高胰岛素血症模型大鼠糖脂代谢的影响。方法雄性SD大鼠以高脂饲料喂养4个月建立高胰岛素血症模型,按照体重和空腹胰岛素水平分为高胰岛素血症对照组(HIC)、0.225、0.45和1.35g/kgbw海洋胶原肽干预组,另选以基础饲料喂养4个月的健康雄性SD大鼠作为空白对照组。MCPs干预组大鼠以灌胃方式给予不同剂量的MCPs,HIC和空白对照组给予等体积蒸馏水灌胃。实验期间HIC及MCPs各组继续喂饲高脂饲料,空白对照组喂饲基础饲料,实验周期8周。于实验结束时进行口服糖耐量实验,检测空腹血糖、胰岛素、血脂水平、抗氧化酶活性及血清丙二醛含量,并观察胰岛β细胞的超微结构改变。结果实验结束时,与HIC组比较,各剂量MCPs组大鼠空腹胰岛素、总胆固醇和甘油三酯水平明显下降;0.225和1.35g/kgbw MCPs组大鼠空腹血糖水平明显降低,0.45和1.35g/kgbw MCPs组大鼠OGTT0.5h、2h血糖水平和血糖曲线下面积明显降低;各剂量MCPs组大鼠血清超氧化物歧化酶活性明显升高,1.35g/kgbw MCPs组大鼠血清谷胱甘肽过氧化物酶活性明显提高,而血清丙二醛含量明显减少,同时胰岛β细胞的超微结构得到明显改善。结论MCPs对高脂饲料诱导的高胰岛素血症大鼠糖脂代谢紊乱具有明显的改善作用。
Objective To study the effects of marine collagen peptides (MCPs) on the glucose and lipid metabolism in hyperinsulinemia model rats. Methods Male Sprague-Dawley rats were fed with high-fat diet for 4 months to establish hyperinsulinemia model. Hyperalgesic rats (HIC), 0.225, 0.45 and 1.35 g / kg bw marine collagen peptides were intervened according to body weight and fasting insulin Group, another healthy male SD rats fed with basic diet for 4 months were selected as blank control group. Rats in MCPs intervention group were given different doses of MCPs by intragastric administration. HIC and blank control group were given equal volume of distilled water. During the experiment, HIC and MCPs continued to be fed with high fat diet, blank control group fed with basic diet, the experimental period was 8 weeks. Oral glucose tolerance test was conducted at the end of the experiment. Fasting blood glucose, insulin, blood lipid levels, antioxidant enzyme activities and serum MDA levels were measured. The ultrastructural changes of islet β cells were observed. Results At the end of the experiment, compared with HIC group, fasting insulin, total cholesterol and triglyceride levels were significantly decreased in MCPs group; fasting blood glucose levels were significantly decreased in 0.225 and 1.35g / kg bw MCPs groups, while fasting blood glucose levels in 0.45 and 1.35g / The serum glucose level and the area under the blood glucose curve of OGTT 0.5h and 2h were significantly decreased in the MCP-treated rats, and the serum SOD activity was significantly increased in the MCPs-treated rats at the dose of 1.35g / kg bw. The levels of glutathione Peroxidase activity was significantly increased, while the serum MDA significantly decreased, while pancreatic β-cell ultrastructure was significantly improved. Conclusion MCPs can significantly improve the disturbance of glucose and lipid metabolism in hyperinsulinemia rats induced by high fat diet.