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为明确河北省小麦纹枯病发生特点及病菌特征,采用五点取样法调查该病的发生情况,通过细胞核染色、菌丝融合反应和r DNA-ITS序列分析测定196株纹枯病菌群体组成,并比较其对不同小麦品种的致病力差异。结果表明,河北省3个不同生态类型麦区30个监测点均有纹枯病发生,临漳县发病最重,其次是邯郸县和馆陶县,青县发病最轻;纹枯病菌可划分为AG-D、AG-B(0)、AGI、AG-4和AG-5共5种融合群,分别占菌株总数的88.3%、1.5%、5.1%、3.6%和1.5%;采自黑龙港平原区和山前平原区的纹枯病菌对石新828、良星99和邯6172的平均致病力均明显强于冀东平原区;菌株可划分为极强、强、中等和弱4个致病类型,分别占菌株总数的45.92%、33.67%、7.65%和12.76%。表明河北省小麦纹枯病发生普遍,总体呈南重北轻的趋势,纹枯病菌群体组成较简单,以强致病力AG-D融合群为主。
In order to clarify the occurrence characteristics and pathogen characteristics of wheat sheath blight in Hebei Province, the five-point sampling method was used to investigate the occurrence of the disease. The population composition of 196 R. solani isolates was determined by nuclear staining, mycelium fusion reaction and r DNA-ITS sequence analysis , And compared their differences in the virulence of different wheat varieties. The results showed that sheath blight occurred in all 30 monitoring sites in three ecotypes of Hebei Province, with the highest incidence in Linzhang County, followed by Handan County and Guantao County and Qingxian County with the lowest incidence. Rhizoctonia solani Divided into 5 fusion groups of AG-D, AG-B (0), AGI, AG-4 and AG-5, accounting for 88.3%, 1.5%, 5.1%, 3.6% and 1.5% The average pathogenicity of Rhizoctonia solani to Heixian 828, Liangxing 99 and Han 6172 in Heilonggang Plain and Piedmont Plain were significantly higher than that in Jidong Plain. The strains were classified as extremely strong, strong and medium Weak 4 pathogenic types, accounting for 45.92%, 33.67%, 7.65% and 12.76% of the total number of strains, respectively. The results showed that the occurrence of sheath blight of wheat in Hebei Province was widespread and showed a trend of south and north with light. The population of R. solani was relatively simple, and the AG-D cluster was the main cause.