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用苗龄２７ｄ的花生无菌苗的叶节为外植体，在高浓度ＢＡ的培养基中，置于约１８００ｌｘ光照度和（２８±１）℃温度条件下培养１５ｄ左右，可见丛生芽分化，５０ｄ后长出整齐一致的丛生芽．④号培养基每个外植体平均有８．２５个芽，最多可达３０个以上，丛生芽的诱导率达９４．４４％．截取诱导苗单芽，转接至含有ＮＡＡ的根诱培养基中，在同样的条件下，培养６ｄ后有白色根点出现，２８ｄ后，⑦号培养基中的外植体出根率达８２．３５％．把经锻炼后的带根小植株，洗去根部培养基后，移栽入经高温灭菌的无菌土中，置于生化培养箱中培养，培养１５ｄ后，长成５～６ｃｍ的小植株，成活率达２２．０６％．
The leaf segments of sterile seedlings of 27-day-old peanut seedlings were used as explants and cultivated for about 15 days at about 1800lx illumination and (28 ± 1) ℃ culture medium with high concentration of BA, After 50d grow clusters of buds neatly. On the other hand, there were 8.25 buds per explant on the 4th medium, up to 30 or more, and the induction rate of clustered buds reached 94.44%. The single shoots were intercepted and transferred to the root-inducing medium containing NAA. Under the same conditions, white root spots appeared after 6 days of culture. After 28 days, the explants root exudation rate in medium ⑦ reached 82 .35%. After the rooted plantlets were harvested and washed out of the root medium, the plants were transplanted into a sterilized soil autoclaved in high temperature and placed in a biochemical incubator. After 15 days of culture, plantlets of 5 to 6 cm in length were planted , The survival rate of 22.06%.