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目的:建立甘草附子药对总生物碱的含量测定方法方法:采用酸性染料比色法,以乌头碱为对照品,检测波长为415 nm,测定药对中总生物碱的含量。结果:该方法能较为准确地测定出药对中总生物碱的含量;总生物碱的浓度在1.2～6.0μg·ml~(-1)范围内呈良好的线性关系(r=0.999 1),平均回收率100.2%(RSD=2.7%)。结论:该方法灵敏,准确可靠,重复性好,可作为总生物碱的含量测定方法。
OBJECTIVE: To establish a method for the determination of total alkaloids in licorice aconite.METHODS: Aconitine dye colorimetry and aconitine were used as reference materials, the detection wavelength was 415 nm, and the content of total alkaloids was determined. Results: The method was able to determine the total alkaloid content in the drug. The linear relationship between the total alkaloids and the concentration of alkaloids was 1.2 ~ 6.0μg · ml ~ (-1) (r = 0.999 1) The average recovery was 100.2% (RSD = 2.7%). Conclusion: The method is sensitive, accurate and reliable, reproducible, and can be used as a method for determination of total alkaloids.