论文部分内容阅读
以紫叶矮樱叶片为试材,通过薄层层析色谱分析法(TLC)、紫外-可见分光光度法(UV)对其色素种类进行初步推断。通过高效液相色谱法对叶片色素进行进一步验证,并对鉴定出的色素种类进行定量分析。结果表明:紫叶矮樱叶片内含有叶绿素(主要是叶绿素a)、类胡萝卜素,以及矢车菊素(Cyanidin)、天竺葵素、矢车菊素半乳糖苷(Cyanidin-galactoside)以及儿茶素、二氢杨梅酮等单体酚;定量分析发现紫叶矮樱叶片呈色的机制主要是叶绿素与矢车菊素半乳糖苷(Cyanidin-galactoside)和矢车菊素(Cyanidin)2种花青苷共同作用的结果,其相对含量在检测色素总含量中占2.85%,类胡萝卜素、对香豆酸、儿茶素等辅助色素对呈色也起到了一定的辅助作用。
The leaves of Eucalyptus przewalskii were used as materials, and their pigment types were preliminarily inferred by TLC and UV-Vis spectrophotometry. The leaf pigment was further verified by HPLC, and the quantitative analysis of the identified pigment species was carried out. The results showed that the leaves of P. purpurea contained chlorophyll (mainly chlorophyll a), carotenoids, and Cyanidin, pelargonid, Cyanidin-galactoside and catechin , Dihydromyricetin and other monomeric phenols. Quantitative analysis revealed that the main mechanisms of the color development of the leaves of Cymbidium longiflorum were chlorophyll and Cyanidin-galactoside and cyanidin As a result of the interaction, the relative content of the total pigment in the test accounted for 2.85%, and carotenoids, p-coumaric acid, catechin and other auxiliary coloring agents also played a supporting role.