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目的研究上海5岁以下婴幼儿病毒性腹泻的病原学情况。方法采集2012年1月-2013年5月复旦大学附属儿科医院5岁以下腹泻患儿粪便标本160份,对病毒的VP1区等易于分型的特征序列扩增后进行测序比对,检测样本中是否含有诺如病毒(NV)、札如病毒(SAV)、轮状病毒(RV)、星状病毒(ASV)、腺病毒(ADV)以及包括脊髓灰质炎病毒(PV)、柯萨奇病毒(COX)和埃可病毒(ECHO)在内的肠道病毒(EV)。结果 160份腹泻标本中共检出含有上述病毒的样本45份,检出率为28.13%。除ADV外,其他7种病毒均有检出,其中检出NV 23例、SAV 5例、RV A 11例、ASV 3例、COX 2例、PV和ECHO 30各1例,其中1例为NV和ECHO 30混合感染。检出的NV均为GⅡ型,其中78.3%为GⅡ.4型,21.7%为GⅡ.3型;11例RV A中,G3P[8]6例,G2P[4]4例,G1P[8]1例;3例ASV阳性样本中,2例为ASV 1,1例是ASV 5。PVⅡ、ECHO 30、COX A4和COX A9各1例。结论上海地区婴幼儿病毒腹泻的感染致病病毒以NV GⅡ和RV A为主。
Objective To study the etiology of viral diarrhea in infants under 5 years of age in Shanghai. Methods 160 stool specimens from children with diarrhea under 5 years old collected from January 2012 to May 2013 in Fudan University Pediatric Hospital were collected and sequenced. (NV), Sapphire virus (SAV), Rotavirus (RV), Astrovirus (ASV), Adenovirus (ADV) as well as viruses including poliovirus (Coxsackievirus) COX) and echovirus (ECHO), including enterovirus (EV). Results 45 of the 160 samples containing diarrhea were detected with the detection rate of 28.13%. In addition to ADV, seven other viruses were detected, including 23 cases of NV, 5 cases of SAV, 11 cases of RV A, 3 cases of ASV, 2 cases of COX, 1 case of PV and ECHO 30, 1 case of NV And ECHO 30 mixed infection. Among the 11 cases of RV A, 6 cases of G3P [8], 4 cases of G2P [4], and G1P [8] were detected in 11 cases of RV A, 1 case. Of the 3 ASV positive samples, 2 were ASV and 1 were ASV5. PVⅡ, ECHO 30, COX A4 and COX A9 in 1 case. Conclusions Infectious pathogenic viruses of infantile virus diarrhea in Shanghai are mainly NV G Ⅱ and RV A.