论文部分内容阅读
人类和鼠类端粒酶活性在各种永生细胞株和肿瘤中呈正调节,它在永生细胞株和肿瘤组织中能检测到,而在原代细胞株和许多邻近肿瘤正常组织中缺乏。为了了解肿瘤发生期间端粒酶的调节,作者应用Northern印迹法和敏感的PCR为基础的端粒酶检测法分析了端粒酶活化期间近期克隆的端粒酶RNA组分(hTR)水平。结果发现端粒酶活性在原代
Human and murine telomerase activity is positively regulated in a variety of immortal cell lines and tumors. It can be detected in immortal cell lines and tumor tissues but is lacking in primary cell lines and many adjacent tumor normal tissues. To understand telomerase regulation during tumorigenesis, the authors used Northern blotting and a sensitive PCR-based telomerase assay to analyze the recently cloned telomerase RNA component (hTR) levels during telomerase activation. Results found telomerase activity in primary