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(五)冰冻干燥法的介紹 从组织化学的观点看来,用一般的固定及包埋方法是有許多不合适的地方。所以多年以来,許多学者采用了冰冻干燥法以期克服其中的一些缺点。最初在1890年,Altmann氏曾設計了一种方法,这种方法是先将组织冰冻。然后,在硫酸內真空低溫(-15℃)下,使组织脫水,他当时的目的是为了准备一种材料,以便于研究各种不同的固定方法和染色方法对于同一组织的效果如何。为了达到这个目的,他曾将冰冻干燥后
(E) introduction of freeze-drying method From the histochemical point of view, with the general method of fixation and embedding there are many inappropriate places. So many years, many scholars have used freeze-drying method in order to overcome some of these shortcomings. Originally in 1890, Altmann had devised a method that first frozen the tissue. The tissue was then dehydrated under vacuum at low temperature in sulfuric acid (-15 ° C). His purpose at the time was to prepare a material in order to study the effect of various fixation methods and staining methods on the same tissue. In order to achieve this goal, he has been freeze-dried