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应用PCR-DGGE技术研究了雷竹林种植过程中土壤细菌群落结构的变化,并运用冗余分析(RDA)手段提取雷竹林土壤细菌群落多样性的干扰控制因子.结果表明:水稻田改种雷竹后,土壤细菌群落的Shannon指数及丰富度指数均显著增加;长期的集约种植过程对雷竹林土壤细菌群落结构产生了影响,主要细菌种群发生了变化,多样性指数表现为先增加、后期大幅降低的趋势.RDA分析表明,土壤pH、总氮、碱解氮以及速效钾这4个变量能够解释高达76.1%的样本总变异,其中土壤pH对细菌群落结构影响最大,但未达到显著水平,表明长期集约种植的雷竹林土壤细菌群落结构的变化是多种环境因子共同作用的结果.
PCR-DGGE was used to study the changes of soil bacterial community structure during the planting of Phyllostachys praecox, and the redundancy analysis (RDA) method was used to extract the interference control factors of soil bacterial community diversity.The results showed that: The Shannon index and the richness index of soil bacterial community increased significantly. The long-term intensive planting process had an impact on the bacterial community structure, the main bacterial population changed, the diversity index increased at first and then decreased significantly .RDA analysis showed that soil pH, total nitrogen, available nitrogen and available potassium could explain up to 76.1% of total sample variance, of which soil pH had the greatest effect on bacterial community structure but did not reach significant level, indicating that Long-term intensive cultivation of Phyllostachys praecox communities soil bacterial community structure changes are the result of a variety of environmental factors together.