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目的:探讨脆性组胺酸三联体基因(fragile histidine triad,FHIT)蛋白在子宫颈癌及子宫颈上皮内瘤变(cervical intraepithelial neoplasm,CIN)组织中的表达及其意义。方法:应用免疫组织化学链霉素抗生物素蛋白-过氧化物酶链接(SP)法检测35例宫颈癌和58例CIN组织中FHIT蛋白的表达,并以20例正常宫颈组织作正常对照,比较不同组织学分级CIN、不同分化子宫颈鳞癌及正常宫颈组织中FHIT阳性表达率。结果:FHIT蛋白在正常宫颈、CIN和宫颈癌组织中的阳性表达率分别为90.0%、74.1%、25.7%,宫颈癌组织中的表达明显减弱或缺失,从正常宫颈组织→CIN→宫颈浸润癌,FHIT表达呈逐渐降低或缺失的趋势,差异具有统计学意义(P<0.01)。FHIT阳性表达率:CINⅠ~Ⅱ级(84.4%)高于CINⅢ级(61.5%),差异有统计学意义(P<0.05);高分化鳞癌(35.3%)高于中低分化鳞癌(16.7%),差异无统计学意义(P>0.05)。宫颈癌组织中FHIT阳性表达汉族(50.0%)明显高于维吾尔族(10.0%),差异有统计学意义(P<0.05)。结论:FHIT蛋白表达降低或缺失在宫颈癌的发生中起重要作用,FHIT可能可以作为一种新的宫颈癌的早期诊断分子指标。
Objective: To investigate the expression and significance of fragile histidine triad (FHIT) protein in cervical intraepithelial neoplasm (CIN) and cervical cancer. Methods: The expression of FHIT protein in 35 cases of cervical cancer and 58 cases of CIN was detected by immunohistochemical streptavidin-peroxidase linked (SP) method. Twenty normal cervical tissues were used as normal control, The positive rates of FHIT in different histological grade CIN, different differentiated cervical squamous cell carcinoma and normal cervical tissue were compared. Results: The positive expression rates of FHIT protein in normal cervix, CIN and cervical cancer tissues were 90.0%, 74.1% and 25.7% respectively. The expression of FHIT protein in cervical cancer tissues was significantly decreased or absent. From normal cervical tissue → CIN → cervical invasive carcinoma , FHIT expression showed a trend of gradually decreasing or missing, the difference was statistically significant (P <0.01). The positive rate of FHIT was higher in CINⅠ ~ Ⅱ (84.4%) than in CINⅢ (61.5%) (P <0.05), while in well differentiated squamous cell carcinoma (35.3%) was higher than that in moderately poorly differentiated squamous cell carcinoma (16.7%) %), The difference was not statistically significant (P> 0.05). The positive expression rate of FHIT in cervical cancer was significantly higher than that in Uygur (50.0%), the difference was statistically significant (P <0.05). Conclusion: The decrease or deletion of FHIT protein plays an important role in the development of cervical cancer. FHIT may be used as a new molecular marker of early diagnosis of cervical cancer.