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采用固定化酵母使氧化型辅酶Ⅱ生成还原型辅酶Ⅱ，再与胆绿素还原酶粗品偶联催化胆绿素还原为胆红素，经纯化后，含量可达９８％。
Immobilized yeast oxidized coenzyme Ⅱ generated reduced coenzyme Ⅱ, and then coupled with the crude bile chlorin reductase catalytic biliverdin to bilirubin, after purification, the content of up to 98%.