嗜人按蚊细胞系——Ana 104是取材于其Ⅰ龄幼虫培养成功的。培养液以7C199培养基,另加0.4%水解乳白蛋白,0.03%谷氨酰胺、10～20%胎牛血清及适量的青、链霉素和非必需氨基酸配制而成。Ⅰ龄幼虫组织片段在培养液中缓慢地、有节律地收缩,并不断长出生发囊和细胞,达二个半月之久。经传代培养后,约7～10天可长满单层,能维持22天以上。至今已传27代。能适应在L-15、DMEM等培养基中生长。从第9代起,将部分细胞进行液氮冻存,经复苏试验复活成功,生长良好。Ana 104细胞系以上皮型细胞为主,也有梭型细胞,染色体2n=6。动态观察其对氨基酸的需求,发现对谷氨酸、酪氨酸、鸟氨酸、胱氨酸和精氨酸需量较大,并可合成天门冬氨酸等。经电镜观察,未见有病毒和支原体污染。还测试了酯酶同功酶带为3条和羧酸酯酶、乙酰胆碱酯酶的比活力。嗜人按蚊嗜吸人血,是我国重要的媒介蚊种。Ana 104细胞系是继冈比亚按蚊、斯氏按蚊和中华按蚊细胞系建立后,又新增添的1种按蚊细胞系。它将有助于从细胞分子角度对嗜人按蚊进行深入的生物学、营养代谢、细胞遗传、与病原体的关系、杀虫机理及生物工程等方面的研究。 The Anopheles anthropophagus cell line, ANA 104, was successfully cultured from its first instar larvae. The culture medium was prepared from 7C199 medium supplemented with 0.4% hydrolyzed lactalbumin, 0.03% glutamine, 10-20% fetal bovine serum, and appropriate amounts of cyan, streptomycin and non-essential amino acids. The first-instar larvae tissue fragments slowly and rhythmically contracted in the culture fluid and grew into hair follicles and cells for up to two and a half months. After subculture, about 7 to 10 days can be covered with a single layer, can maintain more than 22 days. Has passed 27 generations. Can adapt to the L-15, DMEM growth medium. From the 9th generation onwards, some of the cells were cryopreserved in liquid nitrogen and resuscitated successfully after resuscitation and grew well. Ana 104 cell line mainly epithelial cells, but also shuttle cells, chromosome 2n = 6. Dynamic observation of its demand for amino acids, found on glutamic acid, tyrosine, ornithine, cystine and arginine greater demand, and synthesis of aspartic acid and so on. Observed by electron microscopy, no virus and mycoplasma contamination. Also tested esterase isozyme band 3 and carboxylesterase, acetylcholinesterase specific activity. Anopheles anthrax suck blood, is an important media mosquito species in our country. Ana 104 cell line is an anopheles anopheline cell line added after the establishment of Anopheles gambiae, An.penicillium and An. Sinensis cell lines. It will help to further study the biology, nutrition metabolism, cytogenetics, the relationship with pathogens, insecticidal mechanism and bioengineering of Anopheles anthropophagus from the perspective of cellular and molecular.