论文部分内容阅读
应用反转录多聚酶链反应技术 (ＲＴ ＰＣＲ)及单链长度多态性分析法 (ＳＳＬＰ) ,对 45例胃镜活检取材的胃癌及相应正常组织标本中 ｐ73基因ｍＲＮＡ表达水平及 ｐ73基因微卫星位点的杂合缺失 (ＬＯＨ)进行分析 ,探讨该基因表达水平改变及杂合缺失与胃癌发生、发展的关系。材料
Reverse transcription-polymerase chain reaction (RT PCR) and single-stranded length polymorphism analysis (SSLP) were used to analyze the mRNA expression level of p73 gene and p73 gene microsatellite in 45 specimens of gastric cancer and corresponding normal tissue specimens derived from gastroscope biopsies. The point of heterozygous deletion (LOH) was analyzed to investigate the relationship between changes in gene expression levels and loss of heterozygosity and occurrence and development of gastric cancer. material