论文部分内容阅读
目的观察人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs)移植及联用神经节苷酯GM1治疗新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)的效果。方法 RICE法制备七日龄新生大鼠HIBD模型,随机分为hUCMSCs移植组(n=15)、hUCMSCs+GM1组(n=15),72h后经颈静脉移植等量的hUCMSCs,hUCMSCs+GM1组再腹腔注射GM1,两组均在移植后第31、32、33、34、35天用Morris水迷宫对两组大鼠行为学测试,比较两组大鼠脑功能恢复情况。免疫荧光染色观察DiI标记的hUCMSCs移植后在两组大鼠脑中的分布情况;神经元前体细胞标记DCX特异性染色观察hUCMSCs移植后分化成神经元前体细胞的情况。结果 Morris水迷宫测试:hUCMSCs+GM1组逃避潜伏期明显短于hUCMSCs移植组,差异具有统计学意义(P<0.05)。移植后hUCMSCs能迁徙到脑组织中,并分布于缺血区的小血管旁,能分化成神经元前体细胞。hUCMSCs+GM1组大鼠脑片干细胞分布明显多于hUCMSCs移植组(P<0.05)。结论 hUCMSCs移植对HIBD新生大鼠脑功能有修复作用,联用GM1,有协同作用。
Objective To observe the effect of human umbilical cord mesenchymal stem cells (hUCMSCs) transplantation and ganglioside GM1 treatment on neonatal hypoxic-ischemic brain damage (HIBD). Methods The HIBD model of seven-day-old neonatal rats was prepared by RICE method and divided into hUCMSCs transplantation group (n = 15), hUCMSCs + GM1 group (n = 15), hUCMSCs + GM1 group Intraperitoneal injection of GM1, two groups were in the 31,32,33,34,35 days after transplantation with Morris water maze behavioral test of two groups of rats, the recovery of brain function in both groups were compared. Immunofluorescence staining was used to observe the distribution of DiI-labeled hUCMSCs in the brains of two groups. DCX-specific staining of neuronal precursor cells was used to observe the differentiation of hUCMSCs into neuronal precursor cells. Results Morris water maze test: hUCMSCs + GM1 group evidently shorter evasive latency than hUCMSCs transplantation group, the difference was statistically significant (P <0.05). After transplantation, hUCMSCs can migrate to brain tissue and distribute in small blood vessels in the ischemic area, which can differentiate into neuronal precursor cells. The distribution of stem cells in hUCMSCs + GM1 group was more than that in hUCMSCs transplantation group (P <0.05). Conclusion The transplantation of hUCMSCs can repair the brain function of neonatal rats with HIBD. Combined with GM1, there is a synergistic effect.