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目的:研究补阳还五汤对Dahl盐敏感大鼠介导的高血压前期模型肾脏CaM/NO信号通路的干预作用。方法:选7周龄盐抵抗(SS)与盐敏感大鼠(DS)各20只,SS大鼠随机分为正常组和正常给药组,DS大鼠随机分为模型组和模型给药组。4组大鼠统一在SPF动物实验中心喂养。在1周适应性喂养后,用8%NaCl高盐饲料喂食12d。期间采用智能无创血压计监测大鼠血压,12d后当血压值升至120~139mmHg/80~89mmHg(1kPa=75mmHg)这一范围时,确定为高血压前期大鼠模型。停止高盐喂饲,改为普通饲料喂养,并进行补阳还五汤灌胃治疗。治疗结束后取大鼠左肾组织,荧光定量PCR法检测CaM、eNOS的mRNA表达,westernblot和免疫组化方法对eNOS进行定量和定位检测,ELISA法测定NO含量表达。结果:补阳还五汤可以显著增强CaM和eNOS的mRNA及蛋白含量表达(P<0.05),从而增加肾组织中NO含量(P<0.05)。结论:补阳还五汤可能通过激活CaM/NO信号通路,增加肾内NO浓度,延缓肾内皮细胞功能障碍,从而预防高血压前期患者早期肾脏并发症的发生。
Objective: To investigate the effect of Buyang Huanwu Decoction on renal CaM / NO signal pathway mediated by Dahl salt-sensitive rat model of prehypertension. Methods: Twenty SD rats, salt resistant (SS) and saline control (DS), were randomly divided into normal group and normal control group. DS rats were randomly divided into model group and model group . Four groups of rats were uniformly fed in the SPF Animal Experimental Center. After 1 week of adaptive feeding, 12 days were fed with 8% NaCl high salt diet. The blood pressure was monitored by a smart non-invasive sphygmomanometer. After 12 days, the prehypertensive rat model was established when the blood pressure increased to 120 ~ 139mmHg / 80 ~ 89mmHg (1kPa = 75mmHg). Stop high-salt feed, feed instead of normal feed, and make up the yang huanwu decoction gavage treatment. The rats were sacrificed at the end of treatment. The mRNA expression of CaM and eNOS was detected by fluorescence quantitative PCR. Quantitative and localized eNOS was detected by Western blot and immunohistochemistry. The expression of NO was detected by ELISA. Results: Buyang Huanwu Decoction could significantly enhance the mRNA and protein expression of CaM and eNOS (P <0.05), thereby increasing the content of NO in renal tissue (P <0.05). Conclusion: Buyang Huanwu decoction may prevent the occurrence of early renal complications in prehypertensive patients by activating CaM / NO signaling pathway, increasing intrarenal NO concentration and delaying renal endothelial cell dysfunction.