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目的 :研究痢疾杆菌侵袭蛋白与局部免疫反应的关系 ,为痢疾菌苗的研制提供理论依据。方法 :用表达和不表达侵袭蛋白 (Ipa+ /Ipa-)的两株福氏、宋内氏双价痢疾菌苗经角结膜免疫豚鼠 ,并分别用福氏、宋内氏毒株攻击 ,BA ELISA方法检测豚鼠泪液中特异性抗体升高情况。结果 :发现角结膜免疫后豚鼠泪液中特异性双价抗体水平较对照显著升高 ,差别具有统计学意义 ,Ipa+ 菌苗免疫组宋内氏抗体水平较Ipa-免疫组升高 ,差别具有统计学意义 ;攻击后 ,两免疫组局部抗体呈二次反应变化。结论 :侵袭蛋白的表达可加强痢疾菌苗诱导的局部抗体水平。两株双价痢疾菌苗均有较好的免疫原性。
Objective: To study the relationship between Shigella dysenteriae invasion protein and local immune response and to provide a theoretical basis for the development of dysentery vaccine. Methods: Guinea pigs were immunized with two kinds of Freund’s Schwann’s Salivary double-dysentery bacterin by conjunctiva with Ipa / Ipa- Methods Detection of elevated guinea pig lacrimal fluid specific antibodies. Results: It was found that the specific bivalent antibody in guinea pig lacrimal fluid after horn conjunctival immunization was significantly higher than that in control group, the difference was statistically significant. The level of antibodies in Ipa + vaccine group was higher than that in Ipa-immunized group, the difference was statistically significant Significance; after the attack, two immune groups showed partial antibody changes in the second reaction. CONCLUSION: Invasive protein expression can enhance the local antibody level induced by dysentery bacterins. Two strains of bivalent dysentery vaccine have better immunogenicity.