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本文作者采用菠蘿蛋白酶探讨了一种口服酶吸收的新方法。制备了螢光性染料与菠蘿蛋白酶结合的两种结合物。一种是液体制剂,将2g菠萝蛋白酶加到含有60mgNaHCO_3,100mgNa_2CO_3及500mg氯化5—二甲胺基萘磺醯的蒸溜水中并用机械振摇30分钟混合制成混悬液,所得混合物用离心分离,上清液用蒸溜水透析直到透析液中不显螢光为止。圆体制剂是用相似的方法,加入95%醇使染料标记菠蘿蛋白酶从溶液中沉出。液体和固体染料酶制剂在
The authors used bromelain to explore a new method of oral enzyme absorption. Two conjugates of a fluorescent dye and bromelain were prepared. One is a liquid preparation, and 2 g of bromelain is added to distilled water containing 60 mg of NaHCO 3, 100 mg of Na 2 CO 3 and 500 mg of 5-dimethylamino naphthalene sulphonamide and mixed by mechanical shaking for 30 minutes to prepare a suspension. The resulting mixture is centrifuged , The supernatant dialysis with distilled water until the dialysate was not fluorescent so far. In a similar manner, the round body preparation was precipitated with dye-labeled bromelain from the solution by adding 95% alcohol. Liquid and solid dye enzyme preparations