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临床工作中需要一种测定嗜中性细胞杀菌活力的试验。平皿计数技术操作麻烦费时,用~3H胸腺嘧啶掺入法不仅费时而且技术要求较高,不适于常规实验室应用。作者介绍一种微量测定法,只需少量细胞、操作简便,适于临床应用。嗜中性细胞悬液制备:采用改良Boyum法从全血中分离嗜中性细胞。试管内放入聚蔗糖-泛影葡胺液8 ml,其上放置肝素(100单位)抗凝血液10ml~*,1500×g离心20分钟以去除单核细胞、血小
In clinical work, a test to determine neutrophil bactericidal activity is needed. The cuvette counting technique is cumbersome and time-consuming, with ~ 3H thymidine incorporation method not only time-consuming but also have high technical requirements and are not suitable for routine laboratory applications. The authors describe a micro assay that requires only a small amount of cells and is easy to use and suitable for clinical applications. Neutrophil Suspension Preparation: Neutrophils were isolated from whole blood using a modified Boyum method. Test tube into 8 g of polydextran-meglumine, uggs online bestellen, on which placed heparin (100 units) anticoagulant blood 10ml ~ *, 1500 × g centrifuged for 20 minutes to remove monocytes, small blood