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Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst Ⅰ 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis C pneumonia specific antibodies in sera were also assayed with microimmuno fluoresence (MIF) Results About 28% (49/175) of the patients were PCR positive and 25 7%(45/175) were MIF antibodies positive The accordance rate of the two methods was 91 8% Conclusion It is suggested that the C pneumonia infection was common in this group of patients and the C pneumonia Pst Ⅰ474 specific PCR was sensitive and specific for detecting C pneumonia in pharyngolaryngitis or rhinitis and sinusitis
Objective To assess the prevalence of Chlamydia pneumomia DNA in patients with otolaryngic disease Methods PCR assay was used to detect Chlamydia pneumonia specific Pst I 474 fragment DNA in swabs from patients with acute or subacute pharyngolaryngitis or rhinitis and sinusitis C pneumonia specific antibodies in sera were also assayed with microimmuno fluoresence (MIF) Results About 28% (49/175) of the patients were PCR positive and 25 7% (45/175) were MIF antibodies positive The law rate of the two methods was 91 8% Conclusion It is suggested that the C pneumonia infection was common in this group of patients and the C pneumonia Pst I474 specific PCR was sensitive and specific for detecting C pneumonia in pharyngolaryngitis or rhinitis and sinusitis