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目的探讨咪喹莫特对全层皮肤缺损诱导的兔耳增生性瘢痕模型组织学变化的影响及可能的作用机制。方法 24只新西兰白兔双耳腹面手术切除2.0cm×5.0cm全层皮肤至筋膜,建立兔耳增生性瘢痕模型后,随机分成两组,每组12只,将每只兔左耳分别于伤后每日和隔日应用咪喹莫特,共8周,右耳为空白组(非用药组)。结果与非用药组相比较,经咪喹莫特应用8周后,每日用药组的瘢痕厚度(431.22±92.03)、胶原含量(46.56±19.02)和瘢痕指数(0.36±0.31),TIMP-1(16.64±2.14),α-SMA(3.85±1.26)和PCNA(5.68±1.07)均有显著下降(P<0.05),MMP-1(6.71±2.03)显著增加(P<0.05);而隔日用药组,除胶原含量(49.48±17.35)和PCNA(5.33±0.67)有显著下降(P<0.05),MMP-1(5.05±0.76)显著增加(P<0.05)外,瘢痕厚度(621.91±96.81)和瘢痕指数(0.79±0.34),α-SMA(7.13±1.16)和TIMP-1(24.77±4.17)均无明显改变(P>0.05);无论每日还是隔日应用咪喹莫特对TGF-β1的表达均无影响(P>0.05)。结论在创伤早期应用咪喹莫特可降低瘢痕厚度和胶原含量。
Objective To investigate the effect of imiquimod on the histological changes of hypertrophic scars of rabbit ear induced by full thickness skin defect and its possible mechanism. Methods Twenty-four New Zealand White rabbits were surgically excised from the 2.0 cm × 5.0 cm full thickness skin to the fascia. Rabbit ear hypertrophic scar model was established and divided into two groups at random. Twelve rabbits in each group were divided into two groups, Imiquimod daily and every other day after injury, a total of 8 weeks, the right ear is blank group (non-medication group). Results Compared with non-medication group, the daily thickness of scar tissue (431.22 ± 92.03), collagen content (46.56 ± 19.02) and scar index (0.36 ± 0.31), TIMP-1 (P <0.05); MMP-1 (6.71 ± 2.03) significantly increased (P <0.05); while every other day (16.64 ± 2.14), α-SMA (3.85 ± 1.26) and PCNA (5.68 ± 1.07) (621.91 ± 96.81), except the collagen content (49.48 ± 17.35) and PCNA (5.33 ± 0.67), and the MMP-1 (5.05 ± 0.76) And scar index (0.79 ± 0.34), α-SMA (7.13 ± 1.16) and TIMP-1 (24.77 ± 4.17) (P> 0.05). No effect of imiquimod on TGF-β1 (P> 0.05). Conclusion Imiquimod can reduce scar thickness and collagen content early in trauma.