To investigate the Fe2+ effects on root tips in rice plant, experiments were carded out using border cells in vitro. The border cells were pre-planted in aeroponic culture and detached from root tips. Most border cells have a long elliptical shape. The number and the viability of border cells in situ reached the maxima of 1600 and 97.5%, respectively, at 20～25 mm root length. This mortality was more pronounced at the first 1～12 h exposure to 250 mg/L Fe2+ than at the last 12～36 h. After 36 h, the cell viability exposed to 250 mg/L Fe2+ decreased to nought, whereas it was 46.5% at 0 mg/L Fe2+. Increased Fe2+ dosage stimulated the death of detached border cells from rice cultivars. After 4 h Fe2+ treatment, the cell viabilities were≥80% at 0 and 50 mg/L Fe2+ treatment and were