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目的：探讨胃、肠癌的原发耐药性及多药耐药基因（ｍ ｄｒ１）的肿瘤特异性。方法：以未接受肿瘤化疗的 ３１ 例胃癌和 ２４ 例结、直肠癌患者的癌及癌旁组织为研究对象，以逆转录 Ｐ Ｃ Ｒ 技术和 Ｄｏｔ ｂｌｏｔ 技术进行 ｍ ｄｒ１ 基因 ｍ Ｒ Ｎ Ａ 表达的研究。结果：①未接受化学治疗的胃癌患者的癌组织ｍ ｄｒ１ ｍ Ｒ Ｎ Ａ 呈阳性表达者占 ４８３９％ （１５／３１），肠癌为 ５４１７％ （１３／２４）。②癌组织ｍ ｄｒｌ基因ｍ Ｒ Ｎ Ａ 高表达，癌旁组织的表达强度显著低于癌组织。结论：检测癌手术标本ｍ ｄｒ１ 基因可发现原发性耐药，对术后制定化疗方案有重要的参考价值。
Objective: To investigate the primary drug resistance of gastric and colon cancer and the tumor specificity of multidrug resistance gene (m dr1). METHODS: Thirty-one patients with gastric cancer and 24 patients with colorectal cancer who had not received chemotherapy for cancer were studied. The expression of m dr1 gene m R N A was determined by reverse transcription PCR and Dot blot. the study. RESULTS: 1 The positive expression of m dr1 m R N A was 48.39% (15/31) in cancer tissue and 54. 17% (13/24) in gastric cancer without chemotherapy. 2 m drl gene m R N A was highly expressed in cancer tissues, and the expression intensity in adjacent tissues was significantly lower than that in cancer tissues. Conclusion: Detection of mdr1 gene in cancer surgical specimens can find primary drug resistance, and it has important reference value for postoperative chemotherapy.