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研究细胞内组胺在ＰＡＦ诱导血小板活化中的作用．方法：采用血小板聚集及放免法测定ＴＸＢ２，Ｆｕｒａ２负载测细胞内钙．结果：美吡拉敏高于１００μｍｏｌ·Ｌ－１抑制ＰＡＦ诱导的聚集，而西咪替丁在４００μｍｏｌ·Ｌ－１亦无效，组胺及２ｔｈｉａｚｏｌｙｌｅｔｈｙｌａｍｉｎｅ也无协同作用．但在皂素（８－１０ｍｇ·Ｌ－１）通透的血小板，组胺可减弱美吡拉敏的抑制效应，美吡拉敏还可抑制ＰＡＦ引起的ＴＸＢ２产生和细胞内钙增加．结论：ＰＡＦ诱导的血小板活化伴随有细胞内组胺的合成或释放通过ＴＸＢ２产生和细胞内钙增加这一共同通路．
To study the role of intracellular histamine in the PAF-induced platelet activation. Methods: Platelet aggregation and radioimmunoassay TXB2, Fura 2 load measured intracellular calcium. Results: Pymetrozine above 100 μmol·L-1 inhibited PAF-induced aggregation, whereas cimetidine was also ineffective at 400 μmol·L-1. Histamine and 2thiazolylethylamine also showed no synergistic effect. However, platelets with saponin (8-10 mg · L-1) permeabilized showed that histamine attenuated the inhibitory effect of mepyramine, while mepyramine also inhibited PAF-induced TXB2 production and intracellular calcium increase. CONCLUSION: PAF-induced platelet activation is accompanied by intracellular histamine synthesis or release through the common pathway of TXB2 production and increased intracellular calcium.