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应用胞浆分裂阻断微核法，以抗着丝粒血清标记微核（ Ｍ Ｎ），针对血清各种微量营养素对两种自发染色体损伤，即整条染色体丢失和染色体断裂的影响进行了研究。方法：选择 ３３ 名 １９～６９ 岁未使用过任何已知和可疑诱变剂、在此之前 ８ 个月内未进行过 Ｘ 射线检查的健康非吸烟者。取空腹血分离淋巴细胞进行培养，４４ 小时时加松胞素 Ｂ，终浓度为 ３０μｇ／ｍ ｌ，培养至 ７２ 小时，经细胞涂片、干燥、固定并染色。将 Ｔｒｉｓ 缓冲液按 １／８ 稀释的抗着丝粒血清加入到固定了细胞的玻片上，于 ５℃湿盒内孵育过夜，冲洗后加入过氧化物酶标记的兔抗人 Ｉｇ Ｇ，于室温孵育 ３ 小时。用改进的氯化镍／咪唑标准二氨基联苯胺反应进行组化检查。甲基绿复染。计数１ ０００ 个双核细胞确定微核细胞（ Ｍ Ｎ Ｃ）率。至少计数 ２７ 个 Ｍ Ｎ 确定着丝粒（＋ ）和（－ ）比例。血清从全血中分离，测量其中β胡萝卜素、维生素 Ｂ１２ 、维生素 Ｃ、叶酸和维生素 Ｅ等微量营养素含量。结果：简单相关分析表明，年龄与 Ｍ Ｎ Ｃ 率的增加显著相关。与年龄相关增加的 Ｍ Ｎ Ｃ 既包含着丝粒（＋ ），也包含着丝粒（－ ）的 Ｍ Ｎ。除维生素 Ｂ１２与着丝粒（＋ ） Ｍ Ｎ Ｃ 率呈弱正?
The effects of serum micronutrients on two kinds of spontaneous chromosomal damage, namely the whole chromosome loss and chromosome rupture, were studied by applying cytoskeletal blockage micronuclei method and anti-centromere-labeled micronuclei (M N) . METHODS: Twenty-three healthy non-smokers, aged 19-69 who had not used any known and suspected mutagens, and who had not had an X-ray examination within the preceding 8 months, were enrolled. The fasting blood was collected for lymphocyte separation. Cytokinin B was added 44 hours later, and the final concentration was 3 0μg / ml. The cells were cultured to 72 hours. The cells were smeared, dried, fixed and stained. The Tris buffer was diluted 1/8 with anti-centromeric serum to the cell-mounted slide and incubated overnight at 5 ° C in a wet box. After washing, peroxidase-labeled rabbit anti-human Ig G was added and incubated at room temperature Incubate for 3 hours. Histochemical examination was performed with the improved nickel chloride / imidazole standard diaminobenzidine reaction. Methyl Green counterstain. Counting 1,000 dinuclear cells determines the rate of micronuclei (MNC). Count at least 27 M N to determine the centromere (+) and (-) ratio. Serum was separated from whole blood and the levels of micronutrients such as β-carotene, vitamin B12, vitamin C, folic acid and vitamin E were measured. Results: Simple correlation analysis showed that age was significantly associated with an increase in M N C rate. The age-dependent increase of MNC includes both centromere (+) and centromere (-) MN. In addition to vitamin B12 and centromere (+) M N C rate was weak positive?