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为了探索日本血吸虫虫卵的化学致癌性,我们分析了虫卵中含有的某些色氨酸衍生物。虫卵匀浆抽提物经双向纸层析分离,观察到有一个斑点,其Rf值与Ehrlich试验显色,以及斑点洗脱液的可见、紫外和荧光光谱均与标品吲哚相同,表明虫卵中含有吲哚。实验进而利用检测化为物质致突变性╱致癌性的UDS检测法,对吲哚进行检测。吲哚浓度为10~(-4)~10~(-6)M时能诱发FL细胞的UDS。结果表明,虫卵中含有DNA损伤剂吲哚,提示虫卵中存在着促进肿瘤发生的化学因素。
To explore the chemical carcinogenicity of Schistosoma japonicum eggs, we analyzed some tryptophan derivatives contained in the eggs. Egg egg homogenate extracts were separated by bidirectional paper chromatography and a spot was observed. The Rf value and Ehrlich test showed that the visible, ultraviolet, and fluorescence spectra of the spotted elution were the same as the labeled indole, indicating Egg contains indole. The experiments then utilized the UDS assay, which detected the mutagenicity / carcinogenicity of the substance, to detect indole. Indole concentration of 10 ~ (-4) ~ 10 ~ (-6) M can induce FL cell UDS. The results showed that the insect eggs contain DNA damage agent indole, suggesting that there are chemical elements that promote tumorigenesis in eggs.