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目的研究不同类型n-3多不饱和脂肪酸对大鼠脂联素及血脂、血糖水平的影响。方法 40只雄性Wistar大鼠按体质量随机分成正常脂肪供能比(27%)NF1和NF2,高脂肪供能比(42%)HF1和HF2,共4组。NF1和HF1组饲料添加二十碳五烯酸(EPA)和二十二碳六烯酸(DHA),NF2和HF2组饲料添加α-亚麻酸(ALA),喂养12 w。12 w测定肾周脂肪脂联素m RNA,测定0和12 w血清脂联素,0、4、8和12 w检测血脂和血糖。结果 0 w大鼠各指标无组间差异。12 w,NF1组肾周脂肪脂联素m RNA明显高于NF2(P<0.05)。NF1和NF2两组大鼠血清脂联素、高密度脂蛋白胆固醇(HDL-C)较0 w均明显升高(P<0.05),低密度脂蛋白胆固醇(LDL-C)均明显降低(P<0.05),NF1较NF2组作用更显著(P<0.05)。NF1组总胆固醇(TC)较0w显著下降(P<0.05)。HF1和HF2两组大鼠脂联素m RNA明显低于NF1组(P<0.01),血清脂联素和HDL-C也明显降低(P<0.05),空腹血清血糖、LDL-C、TC和甘油三酯(TG)显著升高(P<0.05)。结论相对于ALA,正常脂肪供能比时EPA+DHA更能促进大鼠肾周脂肪脂联素m RNA表达,提高血清脂联素和HDL-C含量,降低LDL-C和TC含量。高脂条件下两者作用无差异。
Objective To investigate the effects of different types of n-3 polyunsaturated fatty acids on adiponectin, blood lipid and blood glucose in rats. Methods Forty male Wistar rats were randomly divided into four groups according to body weight: normal fat supply ratio (27%) NF1 and NF2, high fat supply ratio (42%) HF1 and HF2. Eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were added to the NF1 and HF1 diets, and α-linolenic acid (ALA) was fed to the NF2 and HF2 diets for 12 weeks. Peritoneal adiponectin m RNA was determined at 12 weeks and serum adiponectin levels were measured at 0 and 12 weeks. Serum lipids and blood glucose were measured at 0, 4, 8 and 12 weeks. Results 0 w rat no indicators of differences between groups. At 12 weeks, adiponectin mRNA in perirenal adipose tissue in NF1 group was significantly higher than that in NF2 group (P <0.05). Serum levels of adiponectin and high density lipoprotein cholesterol (HDL-C) increased significantly (P <0.05) and LDL-C in both NF1 and NF2 groups decreased significantly (P <0.05), NF1 was more significant than NF2 (P <0.05). Total cholesterol (TC) in NF1 group decreased significantly compared with 0w (P <0.05). The adiponectin m RNA of HF1 and HF2 groups was significantly lower than that of NF1 group (P <0.01), and the levels of serum adiponectin and HDL-C were also significantly lower (P <0.05). The fasting blood glucose, LDL-C, Triglyceride (TG) was significantly higher (P <0.05). CONCLUSIONS: Compared with EPA, DHA can promote adiponectin m RNA expression in perirenal adipose tissue, increase serum adiponectin and HDL-C, and decrease LDL-C and TC in normal adipose tissue compared with ALA. No difference in the role of both high-fat diet.