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目的改进和提高现行人用地鼠肾细胞狂犬病灭活疫苗的纯度和保护效价。方法 将原代地鼠肾细胞 (PHKC)培养并收获的aG株狂犬病毒原液超滤浓缩 40～ 5 0倍 ,再经Sepharose 6FastFlow分子筛柱层析 ,收集完整的病毒颗粒 ,除菌、灭活、添加Al (OH) 3 佐剂制成纯化地鼠肾细胞狂犬病毒疫苗。结果 浓缩狂犬病毒经过柱层析纯化 ,抗原比活性较纯化前平均提高 2 9 5 4倍 ,纯化效果平均达到 96 1% ,疫苗效力平均达到 2 98IU/ml,其余检测结果均符合现行有关规程。结论 凝胶分子筛柱层析技术 ,是一种快捷而经济的改进现行狂犬疫苗质量的有效方法
Objective To improve and enhance the purity and protective potency of the inactivated human rabies virus inactivated hamster kidney cells. Methods The primary cultured rat kidney cells (PHKC) were harvested and the aG strains of rabies virus were concentrated by ultrafiltration for 40 ~ 50 times. Then the whole virus particles were collected by Sepharose 6 Fast Flow molecular sieve column chromatography and sterilized, inactivated, The purified hamster kidney cell rabies vaccine was made by adding Al (OH) 3 adjuvant. Results The recombinant rabies virus was purified by column chromatography. The specific activity of the recombinant rabies virus was 295 times higher than that before purification, the average purification efficiency reached 96.1%, and the vaccine efficacy reached an average of 2 98 IU / ml. The rest of the results were in accordance with the relevant regulations. Conclusion The gel column chromatography is a fast and economical method to improve the quality of current rabies vaccine