论文部分内容阅读
目的:探讨健脾方对动脉粥样硬化(AS)Apo E基因敲除(Apo E-/-)小鼠心脏和血清5-羟色胺(5-HT)和5-羟吲哚乙酸(5-HIAA)表达的影响,进而明确其作用机制。方法:将Apo E-/-小鼠随机分为模型组,西药对照组(阿托伐他汀),健脾方低、中、高剂量组,饲以高脂饲料,每组10只;将同系C57BL/6小鼠随机分为空白对照组,饲以普通饲料,单纯药物干预组(健脾方中剂量),饲以高脂饲料,每组10只。空白对照组和模型组予0.9%氯化钠溶液灌胃,其余组给予同等剂量的对应药物灌胃,12周灌胃结束后,各组采用ELISA法测定心脏和血清5-HT、5-HIAA含量。结果:与空白对照组比较,模型组5-HT、5-HIAA含量显著升高(P<0.01),5-HT的转化率显著下降(P<0.01);而与模型组比较,各治疗组5-HT和5-HIAA水平显著下降(P<0.01),5-HT转化率也显著降低(P<0.01)。结论:Apo E-/-小鼠早期AS的形成可能与5-HT的代谢减弱使心脏与血清中5-HT高表达有关;健脾方干预早期AS的机制可能与降低心脏和血清中5-HT、5-HIAA的含量有关。
Objective: To investigate the effects of Jianpi Decoction on heart and serum 5-hydroxytryptamine (5-HT) and 5-HIAA (5-HTA) in atherosclerotic Apo E gene knockout ) Expression, and then clarify its mechanism of action. Methods: Apo E - / - mice were randomly divided into model group, western medicine control group (atorvastatin), Jianpifang low, medium and high dose group, fed with high fat diet, each group of 10; C57BL / 6 mice were randomly divided into blank control group, fed with normal diet, simple drug intervention group (Jianpi Fang medium dose), fed with high-fat diet, 10 mice in each group. The blank control group and the model group were given 0.9% sodium chloride solution for gavage, the rest were given the same dose of the corresponding drug gavage, after 12 weeks of gavage, each group using ELISA to measure the serum and serum 5-HT, 5-HIAA content. Results: Compared with the blank control group, the content of 5-HT and 5-HIAA in the model group was significantly increased (P <0.01) and the conversion rate of 5-HT was significantly decreased (P <0.01) 5-HT and 5-HIAA levels were significantly decreased (P <0.01), 5-HT conversion rate was also significantly reduced (P <0.01). CONCLUSIONS: The early formation of AS in Apo E - / - mice may be related to the weakened metabolism of 5-HT and the high expression of 5-HT in serum. The mechanism of intervention of Jianpi prescription in early AS may be related to the decrease of 5- HT, 5-HIAA content.