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目的探讨先天性弓形虫感染的产前诊断方法和防治措施，以减少畸形儿出生。方法采用酶联免疫试验（ELISA）方法筛查5164例孕妇血清，凡TOX-IgG（＋）或TOX－IgM（＋）的孕妇采用聚合酶链反应（PCR）技术检测血TOX－DNA。TOX－DNA（＋）者的孕妇于孕期检测羊水TOX－DNA、AFP和GGT，同时结合B超检查和妊娠史等综合评定，对先天性弓形虫感染作出产前诊断，以采取药物治疗或引产等适当措施。结果5164例孕妇血清中TOX－DNA（＋）30例（0．58％），其中21倒于孕中期接受羊水检测，羊水TOX－DNA（＋）12例，TOX－DNA（－）9例，追踪随访这21例胎（婴）此状况，（引）产后取脐血或畸形部位组织检测TOX－DNA，均与产前诊断相符。结论采用特异性免疫抗体检测和弓形虫基因检测，并结合羊水监测、B超检查等综合评定方法是先天性弓形虫感染较客观的诊断手段，恰当的药物治疗或引产可有效地减少畸形儿出生。
Objective To investigate prenatal diagnosis of Toxoplasma gondii infection and prevention and treatment measures to reduce the birth of deformed children. Methods Serum samples of 5164 pregnant women were screened by enzyme-linked immunosorbent assay (ELISA). TOX-DNA was detected by polymerase chain reaction (PCR) in all TOX-IgG (+) or TOX-IgM (+) pregnant women. Pregnant women with TOX-DNA (+) test for amniotic fluid TOX-DNA, AFP and GGT during pregnancy and prenatal diagnosis of Toxoplasma gondii infection in combination with B-ultrasound and pregnancy history to take medication or induction of labor And other appropriate measures. Results The serum levels of TOX-DNA (+) in 5164 pregnant women were 30 cases (0.58%), of which 21 were pregnant in the second trimester receiving amniotic fluid test. Twelve cases of amniotic fluid TOX-DNA and 9 cases of TOX- Follow-up of these 21 cases of fetal (infants) this situation, (cited) postpartum umbilical cord blood or abnormal tissue samples detected TOX-DNA, are consistent with prenatal diagnosis. Conclusion The detection of specific antibodies and the detection of Toxoplasma gondii, combined with amniotic fluid monitoring, B-ultrasound and other comprehensive assessment of congenital Toxoplasma gondii infection is an objective diagnostic tool, the appropriate drug treatment or induction of labor can effectively reduce the birth of deformed children .