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目的制备垂盆草凝胶剂并对其质量进行控制。方法采用正交设计法优选垂盆草凝胶剂最佳处方,并采用HPLC法同时测定垂盆草凝胶剂中槲皮素、山柰素、异鼠李素含量。结果垂盆草凝胶剂最佳处方为:垂盆草鲜汁40.0g,薄荷油5.0g、卡波姆-940 1.5 g,丙二醇25.0g和25%三乙醇胺20.0g。槲皮素、山柰素和异鼠李素分别在2.632~26.32μg/m L,1.056~10.56μg/ml,0.968~9.68μg/ml范围内呈良好线性关系,相关系数分别为0.9998,0.9997,0.9994。平均加样回收率分别为100.66%,100.45%,97.12%(RSD值分别为0.64,0.77,1.37,n=5)。在该处方条件下制备的三批样品中槲皮素、山柰素、异鼠李素总含量分别为6.72,6.61,6.42μg/g。结论该凝胶剂工艺合理,含量测定方法操作简便,快速准确。
Objective To prepare sachet gels and to control their quality. Methods The orthogonal design was used to optimize the optimal formulation of sodagrast gel, and the contents of quercetin, kaempferol and isorhamnetin were determined simultaneously by HPLC. Results The optimal prescription of sodag gel was as follows: 40.0g fresh sod grass juice, peppermint oil 5.0g, carbomer 940 1.5g, propylene glycol 25.0g and 25% triethanolamine 20.0g. Quercetin, kaempferol and isorhamnetin showed good linearity in the range of 2.632 ~ 26.32μg / ml, 1.056 ~ 10.56μg / ml and 0.968 ~ 9.68μg / ml respectively, the correlation coefficients were 0.9998,0.9997, 0.9994. The average recoveries were 100.66%, 100.45% and 97.12%, respectively (RSD values were 0.64, 0.77 and 1.37, respectively, n = 5). The total contents of quercetin, kaempferol and isorhamnetin in three batches of samples prepared under the prescription conditions were 6.72, 6.61 and 6.42 μg / g, respectively. Conclusion The gel process is reasonable, the content determination method is simple, fast and accurate.