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本实验采用优化的方法通过SPDP交联抗CD_7单克隆抗体与蓖麻毒素制成免疫毒素。经MTT方法检测其对表达CD_7抗原的Molt-4及HPB-ALL细胞系均有显著特异性杀伤作用。克隆杀伤实验结果表明,该免疫毒素在10~(-10)mol/L浓度时达3个杀伤log(即清除99.9%的molt-4细胞),而在此浓度时,其只抑制21.6%的粗-单系造血祖细胞生长,从而初步证明其在自体骨髓移植中可用于清除骨髓中残留白血病细胞。
In this experiment, an optimized method was used to cross-link anti-CD_7 monoclonal antibody with SPDP to make immunotoxins with ricin. The results of MTT assay showed that the MTT assay showed significant killing effect on Molt-4 and HPB-ALL cell lines expressing CD7 antigen. The results of the cloning and killing tests showed that the immunotoxin reached 3 kill log (ie, 99.9% of the molt-4 cells was cleared) at a concentration of 10-10 mol / L, whereas at this concentration, it only inhibited 21.6% Rough - monogenic hematopoietic progenitor cell growth, which preliminary evidence of their autologous bone marrow transplantation can be used to remove bone marrow residual leukemia cells.