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采用同向电渗毛细管电泳方法,使用了亲水性阳离子聚合物聚(2-羟丙基-1,1-N-二甲胺盐酸盐)作为缓冲溶液添加物,显著地减少了分离和测定的时间.为了对分析物得到理想的分离,对分离产生影响的因素包括缓冲溶液添加剂、pH值、缓冲电解质溶液浓度以及分离电压和温度进行逐一优化.发现阳离子聚合物的体积分数在0.000 2%以上都可以使电渗流方向发生反转.pH值、缓冲电解质溶液浓度以及分离电压都对分析物的迁移行为产生影响,而阳离子聚合物的浓度对分析物的分离行为没有影响,也未观察到缓冲溶液添加物与分析物之间相互作用.选择pH 6.65,磷酸盐浓度5 mmol/L,添加体积分数0.2%的阳离子聚合物,25 kV,25℃作为优化的分离条件.建立的分离方法用于中药有效成分的分离和测定,在选择的浓度范围内,峰面积和浓度之间呈良好的线性关系(相关系数为0.9972~0.9977),回收率为92.0%~106.0%.
Using the same method of capillary electrophoresis, poly (2-hydroxypropyl-1,1-N-dimethylamine hydrochloride), a hydrophilic cationic polymer, was used as a buffer solution to significantly reduce the separation and For the ideal separation of the analytes, the factors influencing the separation include buffer solution additives, pH, buffer electrolyte concentration, and separation voltage and temperature, one by one, and the volume fraction of cationic polymer was found to be between 0.0002 % Can reverse the direction of electroosmotic flow.The values of pH, concentration of buffer electrolyte and separation voltage all have an effect on the migration behavior of the analytes, while the concentration of the cationic polymer has no effect on the separation behavior of the analytes, nor is it observed The interaction between the buffer solution and the analyte was optimized, pH 6.65, phosphate concentration 5 mmol / L, cationic polymer 0.2%, 25 kV, 25 ℃ as the optimal separation conditions For the separation and determination of the active ingredients of traditional Chinese medicine, there was a good linear relationship between the peak area and concentration within the selected concentration range (correlation coefficient 0.9972-0.9777). The recovery rate 92.0% and 106.0%.