采用深180毫米以上的普通家用铝锅代替小高压锅进行试管培养基灭菌,和利用自然温度或土法调温代替恒温箱进行母种培养,我已经有六年的实践了,并已在我县举办的多期食用菌培训班中逐步推广。通过生产实践,学员们觉得此法简便易行,菌种质量可靠,适于广大农民利用农余时间,不添设备就能搞好食用菌生产。下面以马铃薯葡萄糖培养基为例,叙述具体做法。把马铃薯洗净,挖去芽眼,去皮,称取200克,切成薄片,在1000毫升水中煮沸20—30分钟,过滤,在清液中加入琼脂15—20克,煮至琼脂溶化,然后加入葡萄糖20克,再补热水达1000毫升,加连霉素3万国际单位,以防细菌污染。放连霉素后一定要搅拌均匀,然后分装试管,塞好棉塞。每7支试管一把,用2—3层牛皮纸包裹管口,用线绳扎成小捆,竖直放入盛有水的铝锅内。在试 Instead of using a small autoclave to sterilize the test tube medium using a regular household aluminum pan with a depth of 180 mm or more, and using natural temperature or soil temperature instead of a thermostat to incubate the mother, I have been practicing for six years and have been with me County organized multi-phase edible mushroom training courses gradually promoted. Through the practice of production, trainees think this method is simple and easy, the strain quality is reliable, suitable for the majority of farmers to use their spare time, without adding equipment can do a good job of mushroom production. The following potato glucose medium, for example, describes the specific practices. The potato was washed, dug the bud eye, peeled, weighed 200 grams, cut into thin slices, boiled in 1000 ml of water for 20-30 minutes, filtered, the agar 15-20 grams in the clear liquid, cook until agar melted, and then Add 20 grams of glucose, and then make up to 1000 ml of hot water, add 30 000 international units of thiamine to prevent bacterial contamination. After letomycin must be mixed evenly, and then packed tube, stuffed tampons. Each one of the test tubes, wrapped with 2-3 layers of kraft paper pipe, tied with a small rope tied into a vertical, filled with water, aluminum pan. In the test