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为研究冻精损伤与精子膜脂质过氧化反应的关系，对１０ 例正常自愿者精液作冷冻处理，检测冷冻前后精液脂质过氧化物（ Ｌ Ｐ Ｏ） 的水平变化及在冷冻剂中加入抗氧化剂 Ｖｉｔ Ｃ，评估冻精活动率、存活率是否改善。 Ｌ Ｐ Ｏ测定采用大石诚子法；精子活动率、存活率采用常规方法检测。结果冷冻前后精液 Ｌ Ｐ Ｏ 水平无显著性差别（ Ｐ＞０ ．０５） ， Ｌ Ｐ Ｏ 含量与冻精活动率、存活率没有相关性；抗氧化剂组与对照组比较，冻精活动率、存活率亦无显著性差别（ Ｐ＞ ０ ．０５） 。结果提示冻精损伤与膜脂质过氧化反应无关。
In order to study the relationship between sperm damage and sperm membrane lipid peroxidation, 10 normal volunteers were frozen, the changes of sperm lipid peroxidation (L P O) level before and after freezing and the addition of Antioxidant Vit C, assess sperm activity, survival rate is improved. L P O determination using the big stone Chengzi method; sperm motility, survival rate by conventional methods. Results There was no significant difference in L P O level between before and after freezing (P> 0.05). There was no correlation between L P O content and activities of frozen sperm and survival rate. Compared with control group, There was also no significant difference (P> 0.05). The results suggest that the injury of frozen sperm has nothing to do with lipid peroxidation.