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目的:以ApoE基因缺陷小鼠为动脉粥样硬化模型,观察有氧运动对小鼠动脉粥样硬化斑块的作用,及运动对血浆一氧化氮(nitric oxide,NO)、血浆L-精氨酸(L-arginine,L-Arg)与不对称二甲基精氨酸(asymmetricdimethlarginine,ADMA)比值的影响,以探讨NO及其底物L-Arg在运动抗动脉粥样硬化中的作用。方法:ApoE基因缺陷小鼠随机分为安静对照组和运动组(游泳运动,2 h/次,5次/周,共10周),分别测定粥样斑块面积,采用硝酸还原酶法测定血浆中NO水平,利用高效液相法测定血清中ADMA与L-Arg的水平,另对血清肌酐和肾脏超微结构进行观察。结果:与对照组相比,运动组小鼠斑块面积显著减小40%(P<0.01),小鼠血浆NO水平升高15倍(P<0.01),L-Arg/ADMA比值显著升高10%(P<0.05)。结论:有氧运动使血浆L-Arg/ADMA比值升高,促进L-Arg的利用,NO合成增多,可能是运动抗动脉粥样硬化的机制之一。
OBJECTIVE: To investigate the effect of aerobic exercise on atherosclerotic plaque in ApoE-deficient mice and the effect of exercise on plasma nitric oxide (NO), plasma L-arginine (L-arginine, L-Arg) and asymmetric dimethylarginine (ADMA) ratios in order to investigate the role of NO and its substrate L-Arg in anti-atherosclerosis. Methods: ApoE gene-deficient mice were divided into quiet control group and exercise group (swimming exercise, 2 h / time, 5 times / week for 10 weeks). ApoE gene-deficient mice were randomly divided into control group and exercise group In the level of NO, the level of ADMA and L-Arg in serum was measured by HPLC, and the ultrastructure of serum creatinine and kidneys were also observed. Results: Compared with the control group, the plaque area of the exercise group was significantly reduced by 40% (P <0.01), the level of NO was increased by 15 times (P <0.01) and the ratio of L-Arg / ADMA 10% (P <0.05). CONCLUSION: Aerobic exercise increases the L-Arg / ADMA ratio in plasma and promotes the utilization of L-Arg, which may be one of the mechanisms of exercise-induced anti-atherosclerosis.