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目的探讨氟砷联合作用对大鼠骨骼功能的影响。方法以SD大鼠为实验动物,析因设计方法随机分成9组,每组6只,雌雄各半。用氟化钠(NaF)和亚砷酸钠(NaAsO2)灌胃染毒。分别为:对照组、低氟组(简称Flow,5mg/kg)、高氟组(简称Fhigh,20 mg/kg)、低砷组(简称Aslow,2.5 mg/kg)、高砷组(简称Ashigh,10 mg/kg)、FlowAslow组、FlowAshigh组、FhighAslow组、FhighAshigh组。酶联免疫吸附法(Elisa)测定大鼠尿Ⅰ型胶原交联氨基末端肽(UNTX)、血清骨钙素(OCN)、骨涎蛋白(BSP)浓度,双能X线骨密度仪测量大鼠双侧股骨骨密度(BMD)。结果 (1)UNTX随着氟、砷剂量增高而升高,存在剂量-效应关系,且其在FlowAslow、FhighAslow剂量组分别低于两者单独作用时的浓度之和(P<0.01)。(2)BSP、OCN随着氟剂量增高而升高,存在剂量-效应关系;BSP、OCN浓度与砷相关关系不明显(P>0.05)。(3)BMD随着氟剂量的升高而增高,但差异无统计学意义(P>0.05);BMD与砷相关关系不明显(P>0.05);与对照组相比除Aslow、FlowAshigh剂量组BMD略为降低,其余各剂量组BMD均略高于对照组,但其差异均无统计学意义(P>0.05)。结论 (1)单独氟能够促进OCN、BSP浓度的升高,氟或砷单独作用能够促进UNTX浓度的升高,在FlowAslow、FhighAslow剂量组,氟、砷对UNTX表现为拮抗作用,UNTX可作为评价氟砷暴露骨损伤联合作用的生物学标志。(2)BMD在一定程度上反映氟对骨骼的损害效应,在该暴露水平下,实验大鼠骨损伤作用类型主要表现为骨质硬化。
Objective To explore the effect of fluoride and arsenic on bone function in rats. Methods SD rats were used as experimental animals. The factorial design method was randomly divided into 9 groups, 6 in each group, with half male and one female. With sodium fluoride (NaF) and sodium arsenite (NaAsO2) intragastric administration. (HF, 5 mg / kg), high fluoride (Fhigh, 20 mg / kg), low arsenic (Aslow, 2.5 mg / kg), high arsenic (referred to as Ashigh , 10 mg / kg), FlowAslow group, FlowAshigh group, FhighAslow group, FhighAshigh group. Urinary type Ⅰ collagen cross-linked amino-terminal peptide (UNTX), serum osteocalcin (OCN) and bone sialoprotein (BSP) were measured by enzyme-linked immunosorbent assay (Elisa) Bilateral femur bone mineral density (BMD). Results (1) UNTX increased with the increase of fluoride and arsenic dose, and there was a dose-effect relationship. The concentrations of UNTX in FlowAslow and FhighAslow dose groups were lower than those of the two groups alone (P <0.01). (2) The dose-effect relationship of BSP and OCN increased with the increase of fluoride dose. There was no significant correlation between BSP and OCN concentration and arsenic (P> 0.05). (3) BMD increased with the increase of fluoride dose, but the difference was not statistically significant (P> 0.05); the correlation between BMD and arsenic was not significant (P> 0.05); Compared with the control group, BMD slightly decreased, BMD of other dose groups was slightly higher than that of the control group, but the difference was not statistically significant (P> 0.05). Conclusion: (1) Fluorine alone can promote the concentration of OCN and BSP, and the effect of UNFX alone can promote the increase of UNTX concentration. In FlowAslow, FhighAslow dosage group, fluorine and arsenic can antagonize UNTX, and UNTX can be used as evaluation Biological Marker in Combination with Fluoride - arsenic Exposure Bone Injury. (2) BMD, to a certain extent, reflects the damage effect of fluoride on bone. Under this level of exposure, the type of bone injury in experimental rats is mainly bone sclerosis.